Thy1-gcamp6 transgenic mice

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Webb7 maj 2024 · In a separate set of experiments using intact cranial windows of Thy1-GCaMP6 transgenic mice to avoid the possible trace left by viral injection, we have set out to identify the possible cell loss ... Webb20 jan. 2024 · Transgenic Thy1.2-GCaMP6s mice expressing GCaMP6 slow in some DRG neurons have been previously described (61). As no significant differences were observed between male and female mice,...

Thy1-GCaMP6 Transgenic Mice for Neuronal Population

Webb24 sep. 2014 · We tested three mouse lines for imaging in the visual cortex in vivo and compared their performance to mice injected with AAV expressing GCaMP6. Furthermore, we show that GCaMP6 Thy1... WebbGCaMP6 functional connectivity maps of all 7 individual mice in the imaging cohort, filtered over the delta (0.4–4.0Hz) frequency band. The same canonical network seeds used in Figs Figs44and and55were applied here. Data shown are acquired in the awake state. (TIF) pone.0185759.s002.tif(2.8M) GUID: C405C9D5-751C-4285-B16C-4C0455F11153 black face cloths

Altmetric – Thy1-GCaMP6 Transgenic Mice for Neuronal …

Webb14 feb. 2024 · Thy1-GCaMP6 transgenic mice for neuronal population imaging in vivo. PLoS One. 2014; 9: e108697. Crossref; PubMed; Scopus (285) Google Scholar, Dana et al., 2024. Dana H. Novak O. Guardado-Montesino M. Fransen J.W. Hu A. Borghuis B.G. Guo C. Kim D.S. Svoboda K. Thy1 transgenic mice expressing the red fluorescent calcium … Webb18 okt. 2012 · To generate GCaMP transgenic mice, we used the well-characterized Thy1 promoter to express GCaMPs in neurons. We generated eight founder lines of … Webb23 juli 2024 · For example, Thy1-GCaMP6 mice show stable expression of GCaMP6 in multiple cortical regions across months without apparent toxicity, but only after the third postnatal week (Dana et al., 2014). Similarly, the Ai38 mouse line expresses GCaMP3 when treated with tamoxifen at P7, but showed very low expression at 4 weeks of age … game gear powerback

Imaging Neural Activity Using Thy1-GCaMP Transgenic Mice

WebbFurther, transgenic mice are now commercially available that express GCaMPs in a Cre recombinase dependent fashion. ... Thy1-GCaMP6 transgenic mice for neuronal population imaging in vivo. PLoS ONE 9:e108697. doi: 10.1371/journal.pone.0108697. PubMed Abstract Full Text CrossRef Full Text Google Scholar. Datta, S. R., and … Webb18 okt. 2012 · To generate GCaMP transgenic mice, we used the well-characterized Thy1 promoter to express GCaMPs in neurons. We generated eight founder lines of … gamegear on retroarchWebbCommon Name: GP4.3. These transgenic mice, made by the Genetically-Encoded Neuronal Indicator and Effector ( GENIE) Project, express the green fluorescent calcium indicator, … blackface colleges

"Webb15 mars 2024 · Here we describe five new transgenic mouse lines expressing GCaMP6F (fast) or GCaMP6S (slow) in the central and peripheral nervous system under the control … " - Thy1-gcamp6 transgenic mice

Thy1-gcamp6 transgenic mice

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Webb23 sep. 2024 · Adult (P65-P70) transgenic mice (C57BL/6J-Tg (Thy1-GCaMP6f) GP5.1Dkim/J; The Jackson Laboratory) expressing GCaMP6f under the Thy1 promoter were used. All animals were housed in a temperature-controlled (24 °C) colony room and maintained in a 12-h/12-h light/dark cycle (lights on at 7:00 a.m.). Food and water were … WebbFurthermore, we show that GCaMP6 Thy1 transgenic mice are useful for long-term, high-sensitivity imaging in behaving mice. View on publisher site Alert me about new mentions. Twitter Demographics. The data shown below were collected from the profiles of 2 tweeters who shared this research output.

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WebbVariants of GCaMP fluoresce in response to calcium binding and serve as an indicator of neuronal activation. These include Thy1-promoter driven GCaMP6 transgenic lines, Tet-dependent GCaMP6f or GCaMP6s transgenic lines and Cre-dependent GCaMP3, GCaMP5, GCaMP6f or GCaMP6s mouse lines. Webb18 okt. 2012 · These GCaMP transgenic mice allow investigation of activity patterns in defined neuronal populations in the living brain and will greatly facilitate dissecting …

Webb20 aug. 2024 · Thy1-GCaMP6 transgenic mice for neuronal population imaging in vivo. Dana H, Chen TW, Hu A, Shields BC, Guo C, Looger LL, Kim DS, Svoboda K. PLoS One, (9):e108697 2014 MED: 25250714 Functional imaging of hippocampal place cells at cellular resolution during virtual navigation. Dombeck DA, Harvey CD, Tian L ... Webb24 sep. 2014 · We quantified GCaMP6 expression across brain regions and neurons and compared to other transgenic mice and AAV-mediated …

Webb024276 C57BL/6J-Tg(Thy1-GCaMP6f)GP5.5Dkim/J These transgenic mice, made by the Genetically-Encoded Neuronal Indicator and Effector ( GENIE ) Project, express the green … Webb12 apr. 2024 · Venglustat in Thy1-aSyn Mice. Thy1-aSyn (line 61) transgenic mice overexpressing human WT α-synuclein under the Thy-1 promoter were maintained on a mixed C57BL/6-DBA/2 background as described previously. 18, 20 Polymerase chain reaction amplification analysis was performed on ear tissue samples collected both at …

WebbImaging neuronal activity in the central and peripheral nervous systems using new Thy1.2-GCaMP6 transgenic mouse lines These GCaMP6 transgenic lines thus provide useful …

Webb11 okt. 2024 · Transgenic mice expressing the green GECI GCaMP6 are already widely used. Here we present the generation and characterization of transgenic mice … black faced apeWebbThese GCaMP reporter mice can be bred to distinct Cre recombinase driver mice to direct expression of this sensor in unique populations of cells. Concerning the central nervous … blackface clownsWebb4 apr. 2024 · Tg (Thy1-GCaMP6f)GP5.5Dkim. Mutation details : The mouse promoter drives neuron-specific expression of this transgene that includes a mutagenized green fluorescent calcium indicator (GCaMP6f), woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), and bovine growth hormone (bGH) polyadenylation sequence. game gear powers on then offWebb1 jan. 2012 · It is shown that GCaMP6 Thy1 transgenic mice are useful for long-term, high-sensitivity imaging in behaving mice and are shown to be effective for in vivo imaging of genetically defined neuronal populations in vivo. 407 PDF View 2 excerpts, cites background DEVELOPMENT OF A NONINVASIVE CALCIUM IMAGING PROBE game gear redumpWebb7 jan. 2024 · In this study, we describe an experimental procedure for measuring calcium dynamics through dual optical windows above bilateral primary somatosensory corticies (S1) in Thy1-GCaMP6s transgenic mice using 2-photon (2P) microscopy. This method enables recordings and quantifications of neural activity in bilateral mouse brain regions … black-face.comWebbGCaMP3 expressing cells in the ganglion cell layer of Thy1-GCaMP3 transgenic mice before and after optic nerve injury The genetically encoded green fluorescent protein-based calcium sensor, GCaMP, has been used to detect … blackface comicsWebbThe genetically encoded green fluorescent protein-based calcium sensor, GCaMP, has been used to detect calcium transients and report neuronal activity. We evaluated the … blackface datalounge