Phospho-specific antibodies by design
WebThe antibody is phospho-specific and may cross-react slightly with some phospho-serine-containing sequences. By ELISA, it recognizes a wide variety of threonine-phosphorylated … WebMonoclonal Antibody for studying Bad (Ser99) phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-Bad (Ser136) (5D8) Mouse …
Phospho-specific antibodies by design
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WebTreat samples with phosphatase to confirm phospho-specific antibodies only recognize the phosphorylated target in IF. If you suspect problems with fixation or permeabilization, checking with an antibody that has worked for your lab in the past can be helpful in troubleshooting. WebThe antibody ab76009 is a phospho specific antibody. It will detect MYBL2 only when it is phosphorylated at T487. You may need to try the antibody ab61128 which will detect total MYBL2 protein however this ab can not distinguish phospho and non phospho protein. Please note that ab61128, antibody is yet not tested in IHC-P so you may be ...
WebFor a phospho-specific antibody with a single phosphorylation site or two sites that are either juxtaposed or with few intervening amino acids, immunodepletion of the serum before affinity purification may be sufficient to remove cross-reactivity to the unmodified peptide/protein. Dot blots are often a good way to determine potential cross ... WebMonoclonal Antibody for studying Thr-P. Cited in 2 publications. Highly specific and rigorously validated in-house, Phospho-Threonine (42H4) Mouse Monoclonal Antibody (CST #5005) is ready to ship. ... Phospho-Threonine (42H4) Mouse mAb 可用于高通量激酶测定和药物发现应用。 ...
WebHigh-quality phospho-specific antibodies for more than 300 phosphoproteins or phosphorylation sites; Custom special phospho-specific antibodies against every … WebPhospho-Specific Antibodies. Phosphorylation is the addition of a phosphate (PO43−) group to a protein or other organic molecule. Protein phosphorylation is a post-translational modification of proteins in which a serine, a threonine or a tyrosine residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group.
WebThe crucial first step in the development of custom-made phosphospecific antibodies is the selection and design of the immunizing peptide. Western blots are essential to truly establish the specificity of an antibody for the holoprotein of interest. rothiemurchus accommodationWebJan 1, 2011 · As mentioned above, phospho-specific antibodies were first successfully prepared by immunization of rabbits with native purified protein. However, virtually all phospho-specific antibodies are now produced using phosphopeptides as antigens. In using synthetic peptides to produce antibodies, the key step is the design of the antigen. st philips catholic church corpus christiWebPhosphorylation state-specific antibodies provide us with a remarkably sensitive and specific immunochemical tool to detect and quantify changes in the phosphorylation state of particular amino acid residues within the … rothiemurchus army lodgeWebPhospho-specific antibodies Antibodies that discriminate between the phosphorylated and the non-phosphorylated state of a protein, is one of the most frequently asked for types of PTM specific antibodies. These could be made as monoclonal antibodies but are more often produced as antigen specific affinity purified polyclonal antibodies. st philips catholic church linesville paWebJan 23, 2014 · Phospho- and non-phospho-specific antibodies provide unique reagents that allow the analysis of the spatial distribution of protein modifications that would be difficult or impossible to obtain by ... st. philips catholic church falls church vaWebPhospho-specific antibodies, which can discriminate between the phosphorylated and non-phosphorylated forms, have emerged as demonstrated assets in the study of signal … rothiemurchus activity centrehttp://www.innovagen.com/phospho-specific-antibodies st philips catholic church jonesboro ga