Pcdf1b
Splet05. okt. 2024 · The access to information on the dynamic behaviour of large proteins is usually hindered as spectroscopic methods require the site-specific attachment of … Splet17. jul. 2024 · A single colony was then used to inoculate 5 mL of LB medium containing 50 μg/mL streptomycin (only where the pCDF1b-Sfp vector was present) and kanamycin, …
Pcdf1b
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SpletRosetta DE3 pLys. 6xHIS-Tev site-Get5-pCDF1b (23) or 6xHIS-Tev site-Get5*-pCDF1b was co-transformed with Get4-pET28 (23) into E. coli Rosetta DE3 pLys. The bacteria with … SpletPlasmid pAW150 from Dr. Jennifer Doudna's lab contains the inserts Cas1 and Cas2 and is published in Science. 2024 Sep 15;357(6356):1113-1118. doi: 10.1126/science.aao0679. Epub 2024 Jul 20. This plasmid is available through Addgene.
Splet15. jul. 2011 · The 5.5 protein (T7p32) of coliphage T7 (5.5T7) was shown to bind and inhibit gene silencing by the nucleoid-associated protein H-NS, but the mechanism by … Splet07. mar. 2014 · The Haloferax Cas6 protein shows very little sequence similarity to other Cas6 proteins from type I systems and to most systems in general (Fig. 2).As visible in the alignment, only three positions are conserved in all Cas6 proteins analyzed (Gly-122, Gly-258, and Gly-258). Only one amino acid, shown to be important for catalytic activity in …
SpletThe pCDF1b vector was digested with the restriction enzymes XhoI and NdeI, which eliminated the sequence encoding the His6 tag from the vector backbone, thereby … Splet10. maj 2013 · The pCDF1b vector was digested with the restriction enzymes XhoI and NdeI, which eliminated the sequence encoding the His6 tag from the vector backbone, thereby …
Splet08. okt. 2010 · Cultures transformed with each PemK construct also were cotransformed with pCDF1b:PemI or pCDF1b empty vector (Fig. (Fig.5C). 5C). Growth of cotransformed …
Splet14. avg. 2005 · Type I polyketide synthase (PKS) genes consist of modules ∼3–6 kb long, which encode the structures of 2-carbon units in polyketide products. Alteration or replacement of individual PKS ... peanut butter face maskSplet11. nov. 2024 · To create pKB822, the PCR product of primers oKB1044 + oKB1045 on pBAD33 containing araC-pBAD-rrnB was digested with Bsu36I and NarI, and ligated with … peanut butter espresso browniesSpletpAW212载体价格质粒购买价格是1200元，pAW212载体质粒图谱(Vector map)，pAW212 (addgene 113234)载体序列(Sequence)见下文，载体质粒抗性为链霉素，质粒大小为4909 bp。pAW212载体是细菌表达质粒。 lightning glass new braunfelsSpletpCDF1b vector (Merck), CloDF13 ori, T7 promoter, streptomycin resistance gene) [3] to construct pCDF-epa-C_KS C and pCDF-epa-C_CLF. 5 Figure S1. PUFA biosynthetic pathway of PUFA synthases. MAT ACP S O HO O ACP SH ACP S O R O ACP S O R OH ACP S O R ACP S O R ACP S O R R KS KR DHFabAorDHPKS ER NADP+ NADPH NADPH NADP+ CO2 H2O … lightning glass repairSpletCentrifuge at 5,000×g for 5 min. 2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g. 5.Store the plasmid at -20 ℃. peanut butter eyesSpletPlasmid. Description. Metadata. Relevant Items. Editing and analysis saving disabled due to read-only access. Create PDF. pCDF-1b 3621 bp 500 1000 1500 2000 2500 3000 3500 … peanut butter experimentsSpletvectors pET-28a, pCDF1b and pBbE8kRFP 1 were purchased from Novagen (Darmstadt, Germany) and were used for gene expression. Restriction enzymes, T4 ligase, taq … peanut butter facial mask