WebCHX Chase assay. The western blot performed in presence of MG132, after 48 h from transfection showed that the mutated proteins were degraded (a) up to the 80% with respect to the WT, with short ... WebApr 1, 2024 · CHX-chase assay Transfected HEK 293T cells were cultured in presence of 50 μg/ml CHX (Sigma), in some instances in combination with 50 μM MG132 (Calbiochem) for the indicated time periods.
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WebApr 1, 2024 · Each SNAP-GPCR-CHX-chase assay was performed in triplicate, and data were analyzed with GraphPad Prism software to calculate the protein half-life (t 1/2) with … WebMar 20, 2007 · A small N-terminal deletion of KLF5 blocks protein degradation. (A) A 19-amino acid but not 62-amino acid deletion at the N-terminus of KLF5 extends protein half-life as detected by CHX chase assays. WT KLF5 was used as a control. All three constructs have a FLAG tag at the C-terminus. An anti-FLAG Ab was used to examine the protein … can going over your credit limit affect you
A conserved ubiquitin- and ESCRT-dependent pathway internalize…
WebOct 9, 2024 · The cycloheximide (CHX) chase assay showed that the DPBA plus CHX treatment lowered the EGFR protein levels considerably more than the CHX treatment alone (Fig. 2b), indicating that DPBA down ... WebNov 21, 2014 · The c-Myc transcription factor is a key regulator that stimulates cell proliferation, apoptosis and differentiation. 14, 15, 16, 17 Deregulated expression of c-Myc has been observed in a wide... WebCycloheximide can be used as an experimental tool in molecular biology to determine the half-life of a protein. Treating cells with cycloheximide in a time-course experiment followed by western blotting of the cell lysates for the protein of interest can show differences in protein half-life. fit by charro login