WebChIRP, also commonly referred to as ChIRP-seq, is a protocol to detect the locations on the genome where ncRNAs, such as lncRNAs, and their proteins are bound. In this method, … ChiRP-Seq (Chromatin Isolation by RNA purification) is a high-throughput sequencing method to discover regions of the genome which are bound by a specific RNA (or by a ribonucleoprotein containing the RNA of interest). Recent studies have shown that a significant proportion of some genomes (including mouse and human genomes) synthesize RNA that apparently do not code for proteins. The function of most of these non-coding RNA still has to be ascertained. Various geno…
HiChIRP Protocol Reveals How lncRNAs Organize the Genome
WebMar 25, 2012 · This method, Chromatin Isolation by RNA Purification (ChIRP) (Figure 1), is based on affinity capture of target lncRNA:chromatin complex by tiling antisense-oligos, which then generates a map... WebMagna ChIRP™ TERC lncRNA Probe Set (Odd) 50 µM CS216563 12 µL RNA Positive Control Primers (TERC Gene) F: CGC TGT TTT TCT CGC TGA CT R: GCT CTA GAA TGA ACG GTG GAA CS216598 75 µL RNA Negative Control Primers (GAPDH Gene) F: GTC GGA GTC AAC GGA TTT G R: TGG GTG GAA TCA TAT TGG AA CS216610 75 µL … greenhead lane knoxville tn
ChIRP-MS: RNA-Directed Proteomic Discovery - PubMed
WebDec 8, 2024 · We identified a long noncoding RNA (lncRNA), LncMyoD, which regulates lineage determination and progression through modulating chromatin accessibility. Functional analysis showed that loss of … WebMar 28, 2024 · LncRNA 作为重要的调控因子在基因表达和细胞命运选择方面发挥重要功能,其在造血干细胞的干性维持以及各系分化等方面的功能也逐渐被发现[1]。 ... ,并且定位于细胞核内的lncRNA具有参与转录调控的潜在功能,因此作者利用MEL细胞系进行了ChIRP-seq实验[3],对 ... WeblncRNA表达载体 circRNA表达载体 蛋白质表达载体 荧光素酶报告基因载体 Northern blot Marker Southern blot Marker-DIG DNase footprinting Marker-Biotin 信号转导试剂盒 CLIP Kit EMSA试剂盒 Co-IP试剂盒 GST pulldown试剂盒 MeRIP试剂盒 MeDIP试剂盒 Northern Blot试剂盒 细胞检测相关产品 腺病毒 慢病毒 green headlights legal